Y Development Fund plus the Geothermal Study Group (GEORG) Fund.The cell pellets had been collected by centrifugation (1000 ?g for 10 min) at four then extracted with 3ml of ethanol: hexane two:1 (v/v) containing 0.1 (w/v) butylated hydroxytoluene till colorless [34]. Towards the mixed answer, 2 ml deionized water and four ml hexane have been added and the mixture was vigorously shaken and centrifuged once again at 1000 ?g for five min [7]. An aliquot of four ml of the upper hexane layer was evaporated below nitrogen at 25 ?two , reconstituted within a mixture of methyl tertiary butyl ether : acetonitrile (50:50)Fu et al. Microbial Cell Factories 2014, 13:three http://microbialcellfactories/content/13/1/Page 9 ofAuthor facts 1 Center for Systems Biology, University of Iceland, Reykjav 101, Iceland. 2 Division of Bioengineering, University of California, La Jolla, San Diego, CA 92093-0412, U.Buy(R)-(Piperidin-3-yl)methanol S.A. 3Faculty of Industrial Engineering, Mechanical Engineering and Computer Science, University of Iceland, Reykjav 101, Iceland. 4Faculty of Life and Environmental Sciences, University of Iceland, Reykjav 101, Iceland. Received: 27 September 2013 Accepted: 6 January 2014 Published: 8 JanuaryReferences 1. Cordero BF, Obraztsova I, Couso I, Leon R, Vargas MA, Rodriguez H: Enhancement of lutein production in Chlorella sorokiniana (chlorophyta) by improvement of culture circumstances and random mutagenesis. Mar Drugs 2011, 9:1607?624. two. Lamers PP, Janssen M, De Vos RCH, Bino RJ, Wijffels RH: Exploring and exploiting carotenoid accumulation in Dunaliella salina for cell-factory applications. Trends Biotechnol 2008, 26:631?38. three. Pulz O, Gross W: Precious solutions from biotechnology of microalgae. Appl Microbiol Biotechnol 2004, 65:635?48. four. Suh IS, Joo HN, Lee CG: A novel double-layered photobioreactor for simultaneous Haematococcus pluvialis cell growth and astaxanthin accumulation. J Biotechnol 2006, 125:540?46. five. Vilchez C, Forjan E, Cuaresma M, Bedmar F, Garbayo I, Vega JM: Marine carotenoids: biological functions and industrial applications. Mar Drugs 2011, 9:319?33. 6. Del Campo JA, Garcia-Gonzalez M, Guerrero MG: Outdoor cultivation of microalgae for carotenoid production: Current state and perspectives. Appl Microbiol Biotechnol 2007, 74:1163?174. 7. Fu W, Gudmundsson O, Paglia G, Herjolfsson G, Andr son OS, Palsson B? Brynjolfsson S: Enhancement of carotenoid biosynthesis inside the green microalga Dunaliella salina with light-emitting diodes and adaptive laboratory evolution. Appl Microbiol Biotechnol 2013, 97:2395?403. eight. Fernandez-Sevilla JM, Acien Fernandez FG, Molina Grima E: Biotechnological production of lutein and its applications. Appl Microbiol Biotechnol 2010, 86:27?0. 9. Carpentier S, Knaus M, Suh M: Associations involving lutein, zeaxanthin, and age-related macular degeneration: an overview.1450754-38-7 Data Sheet Crit Rev Food Sci Nutr 2009, 49:313?26.PMID:31085260 10. Blanco AM, Moreno J, Del Campo JA, Rivas J, Guerrero MG: Outdoor cultivation of lutein-rich cells of Muriellopsis sp. in open ponds. Appl Microbiol Biotechnol 2007, 73:1259?266. 11. Lesser MP: Oxidative pressure in marine environments: Biochemistry and physiological ecology. Annu Rev Physiol 2006, 68:253?8. 12. Jimenez C, Cossio BR, Rivard CJ, Berl T, Capasso JM: Cell division inside the unicellular microalga Dunaliella viridis is determined by phosphorylation of extracellular signal-regulated kinases (ERKs). J Exp Bot 2007, 58:1001?011. 13. Apel K, Hirt H: Reactive oxygen species: metabolism, oxidative strain, and signal transduction. Annu Rev.
