PKSPKSPKVPF S652 S769 S775 T776 T777 IVTMKRSKSFDDLTTVREK S802 T807 VDDDGTEADDE EDTHPSKEKHL T839 S303 S304 S305 1 1 1 9 KSSTGSPRSKM QNAVSSGDESDIST CDIILSIDDKN INRASSSQNSL VPGNISGDNIF S311 S101 S802 S21 S253 S247 two 1 GKALDSDDEDF LARRSSSQTGFV S423 S247 GSK3 PKA, PKC, CDK1 CAMK2, AKT, CDK1 CK2 GSK3, CAMK2, PKD, CHK1 CK2 CK1, CDK1, MAPK, PKC, GSK3 CK2, GSK3, CK1, DNAPK, ATM, PKC CK2 PKA, CAMK2, PKD, CHK1 S phase Mitosis 1 1 Mitosis S phase S phase 9 7 2 1 S phase three six SHPARSSSFSRIN T829 S800 KKRRRSDGLAL DGLALSTTDLESE S622 S601 DELVMSPIPTT S25 S1354 PKA, PKD, Aurora, CHK1 CAMK2, PKA 1 2 FRKSFSDVRLA ELRIDSSKTLP S1054 CK1, GSK3, CAMK2, PKA, PKG, PKC, CDK5 10 1 9 CK1, CDK1, MAPK SGVLESNLSPKLTS S741 PKCDescriptionPeptide counts Sequence Kinase predictionPhosphoepitopeHighest detection in S or M phase# TM DomainsSignalP PredictionPLOS 1 | plosone.orgYes YesTAHypotheticalTAHypotheticalTAHypotheticalTAABC transporterTAPTATaSPTACation transporting ATPaseTAcdp-diacylglycerol synthaseTAHypotheticalTAHypotheticalTAHypotheticalPhosphorylation of Theileria annulata Schizont Surface ProteinsTAHypotheticalPhosphorylation of Theileria annulata Schizont Surface ProteinsYes/signal anchorSignalP PredictionYes# TM DomainsKinase predictionHighest detection in S or M phase0 CDK1 CK1, PKC, CDKALKCDK1, MAPKYesPhospho-epitopes indicated in bold were these located more abundantly in samples from M- or S-phase (p,0.05). doi:10.1371/journal.pone.0103821.tPhosphoepitopePeptide countsFigure 7. Schematic overview displaying all phosphorylated web sites detected on A) TaSP (TA17315) and B) p104 (TA08425). Detected phosphorylation internet sites are indicated using the amino acid quantity. White circles (P) represent phosphorylation web sites detected in each mitotic and S-phase samples. Grey circles (P) represent phosphorylation web sites using a important distinction in abundance amongst S-phase and mitotic samples. TaSP: schematic topology as predicted by TMpred: 1st TM: 3-21 aa; 2nd TM: 205-223 aa and 3rd TM 262-288 aa. Two phosphorylated serines (S303 and S305) within the Cterminal domain had been extra extremely phosphorylated in S-phase schizontsamples (p,0.01). p104 is predicted to possess a GPI-anchor (GPI). Phosphorylation of four serines (S601, S607, S800 and S802) was significantly improved in S-phase when in comparison to mitosis. doi:10.1371/journal.pone.0103821.gYSSS27 SGLHESSCNSTPREGPKFEASPKLLIKNTEASPKTIMSAKPGYSIIKVSequenceSJNK, and if that’s the case, no matter if this could contribute to preserving the transformed phenotype. In our previous perform we showed by gel shift assay that endogenous p104 is extremely phosphorylated in unsynchronised cell cultures (which primarily consist of cells in G0-G1-phase, Figure S4A), using a slight boost inside the general phosphorylation of p104 detected in mitotic cells [25].1,3,5-Tribromo-2,4,6-trimethylbenzene Formula Because the interaction of a lot of plus finish tracking proteins with EB1 is regulated by phosphorylation inside the vicinity from the SxIP motif, we focused in detail on a short fragment (p104521?34) that encompasses the EB1-binding domain and 21 potentially phosphorylated internet sites (dashed underlined in Figure S4).Price of 1539-42-0 We showed that this short fragment, just like the endogenous protein, is phosphorylated and that in this case the “up-shift” observed in mitotic cells when compared with unsynchronised cells was extremely striking.PMID:28739548 This indicated to us that this region is subjected to in depth cell cycle-dependent regulation of phosphorylation. We went on to show that CDK1 activity is partially,SfiI-subtelomeric fragment associated proteinDescriptiondyn.